TY - BOOK ID - 113295 TI - Differential-display reverse transcription-PCR (DDRT-PCR) AU - Colonna-Romano, Sergio AU - Leone, Antonella AU - Maresca, Bruno PY - 1998 SN - 3540632972 3642804543 9783540632979 PB - Berlin Springer DB - UniCat KW - Analytical biochemistry KW - Gene expression KW - Polymerase chain reaction KW - Reverse transcriptase KW - Research KW - Methodology KW - Biochemical Processes KW - Nucleic Acid Amplification Techniques KW - Gene Expression KW - Chemical Processes KW - Genetic Techniques KW - Biochemical Phenomena KW - Genetic Processes KW - Chemical Phenomena KW - Investigative Techniques KW - Genetic Phenomena KW - Phenomena and Processes KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Polymerase Chain Reaction KW - Transcription, Genetic KW - Biology KW - Health & Biological Sciences KW - Genetics KW - Cell biology. KW - Biochemistry. KW - Cell Biology. KW - Biochemistry, general. KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Chemistry KW - Medical sciences KW - Cell biology KW - Cellular biology KW - Cells KW - Composition KW - Gene expression - Research - Methodology UR - https://www.unicat.be/uniCat?func=search&query=sysid:113295 AB - Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels. ER -