TY - BOOK ID - 11324722 TI - Methods in enzymology. PY - 2009 VL - 459 SN - 00766879 SN - 0123745918 9780123745910 9786612120947 1282120948 0080923364 PB - Amsterdam [Netherlands] ; Boston [Mass.] : Academic Press/Elsevier, DB - UniCat KW - Biosynthesis. KW - Carbohydrates -- Synthesis. KW - Microbial biotechnology. KW - Peptides -- Synthesis. KW - Polyketides -- Synthesis. KW - Metabolism KW - Carbon-Carbon Ligases KW - Multienzyme Complexes KW - Complex Mixtures KW - Biochemical Processes KW - Chemicals and Drugs KW - Enzymes and Coenzymes KW - Chemical Processes KW - Biochemical Phenomena KW - Ligases KW - Metabolic Phenomena KW - Chemical Phenomena KW - Phenomena and Processes KW - Peptide Biosynthesis KW - Polyketide Synthases KW - Enzymes KW - Biological Products KW - Carbohydrates KW - Human Anatomy & Physiology KW - Health & Biological Sciences KW - Animal Biochemistry KW - Peptides KW - Polyketides KW - Synthesis. KW - Peptide synthesis KW - Acetogenins KW - Biological synthesis KW - Synthesis, Biological KW - Ketenes KW - Polymers KW - Biochemical engineering KW - Biochemistry KW - Organic compounds KW - Synthetic biology KW - Biochemical templates KW - Synthesis UR - https://www.unicat.be/uniCat?func=search&query=sysid:11324722 AB - Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been ER -