TY - BOOK ID - 101575922 TI - The P2X7 Receptor : Methods and Protocols PY - 2022 SN - 1071623842 1071623834 PB - New York, NY : Springer US : Imprint: Humana, DB - UniCat KW - Biochemistry. KW - Immune system. KW - Cellular signal transduction. KW - Pharmacology. KW - Immune Cell Signalling. KW - Drug effects KW - Medical pharmacology KW - Medical sciences KW - Chemicals KW - Chemotherapy KW - Drugs KW - Pharmacy KW - Cellular information transduction KW - Information transduction, Cellular KW - Signal transduction, Cellular KW - Bioenergetics KW - Cellular control mechanisms KW - Information theory in biology KW - Immunological system KW - Anatomy KW - Immunology KW - Biological chemistry KW - Chemical composition of organisms KW - Organisms KW - Physiological chemistry KW - Biology KW - Chemistry KW - Physiological effect KW - Composition UR - https://www.unicat.be/uniCat?func=search&query=sysid:101575922 AB - This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors. ER -