TY - BOOK ID - 101480950 TI - Efficient screening techniques to identify mutants with TR4 resistance in banana : protocols AU - Jankowicz-Cieslak, Joanna. AU - Ingelbrecht, Ivan PY - 2022 SN - 3662649152 3662649144 PB - Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, DB - UniCat KW - Plant diseases. KW - Plant genetics. KW - Agriculture. KW - Plant Pathology. KW - Plant Genetics. KW - Farming KW - Husbandry KW - Industrial arts KW - Life sciences KW - Food supply KW - Land use, Rural KW - Plants KW - Genetics KW - Botany KW - Communicable diseases in plants KW - Crop diseases KW - Crops KW - Diseases of plants KW - Microbial diseases in plants KW - Pathological botany KW - Pathology, Vegetable KW - Phytopathology KW - Plant pathology KW - Vegetable pathology KW - Agricultural pests KW - Crop losses KW - Diseased plants KW - Phytopathogenic microorganisms KW - Plant pathologists KW - Plant quarantine KW - Pathology KW - Diseases and pests KW - Diseases KW - Wounds and injuries KW - Musa KW - Fusarium wilt TR4 KW - mutation breeding KW - phenotyping KW - genotyping KW - NGS KW - Bananas KW - Fusarium wilt of banana. KW - Plant mutation. KW - Disease and pest resistance. KW - Genetics. KW - Bananes KW - Patologia vegetal KW - Genètica vegetal KW - Resistència als plaguicides KW - Mutació (Biologia) UR - https://www.unicat.be/uniCat?func=search&query=sysid:101480950 AB - Bananas are a staple food for over 500 million people and are also an important cash crop. Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense, is one of the most destructive diseases of banana globally. Since the 1990s, an aggressive variant of this fungus, called Tropical Race 4 (TR4), severely affected banana plantations in Southeast Asia from where it spread to other continents, including Latin America, where the global banana export market is primarily centred. TR4 is a soil borne pathogen making the disease difficult to contain. The Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture implemented a Coordinated Research Project (CRP) ‘Efficient Screening Techniques to Identify Mutants with Disease Resistance for Coffee and Banana” (2015-2020). This CRP brought together experts from Asia, Europe and Africa in addition to experts of the Joint FAO/IAEA Centre to develop resistance against TR4 through mutation-assisted breeding. Induced mutagenesis is particularly attractive in case of banana since most cultivated bananas are seedless, thus hampering conventional cross breeding. This Open Access book is a compilation of the protocols developed under the CRP specifically for TR4. The first part covers methods for mutation induction, including the integrated use of innovative single-cell culture with mutagenesis techniques. The book also describes up-to-date phenotypic screening methods for TR4 resistance in banana under field-, greenhouse- and laboratory conditions. Finally, molecular and bioinformatics tools for genome-wide mutation discovery following Next Generation Sequencing are also described. Given the imminent threat of Fusarium Wilt TR4 on banana production globally, it is our hope and intention that the book will serve as a timely reference and guide for banana breeders and pathologists worldwide who are committed to the genetic improvement of banana for Fusarium wilt resistance. ER -