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Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.

Polymerase chain reaction --- Diagnostic use. --- Molecular diagnosis --- Life Sciences --- Genetics and Molecular Biology --- Chemical Biology --- Biochemistry --- Bioorganic Chemistry

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This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics.

Human biochemistry --- Medical microbiology, virology, parasitology --- Pathological biochemistry --- Human medicine --- Biochemical engineering --- PCR (polymerase chain reaction) --- medische microbiologie --- medische biochemie --- proteomics --- bio-engineering --- biochemie --- biomedische wetenschappen --- biotechnologie --- moleculaire biologie

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This book highlights the estimation of crop yield in Central Gujarat, especially with regard to the development of Multiple Regression Models and Principal Component Regression (PCR) models using climatological parameters as independent variables and crop yield as a dependent variable. It subsequently compares the multiple linear regression (MLR) and PCR results, and discusses the significance of PCR for crop yield estimation. In this context, the book also covers Principal Component Analysis (PCA), a statistical procedure used to reduce a number of correlated variables into a smaller number of uncorrelated variables called principal components (PC). This book will be helpful to the students and researchers, starting their works on climate and agriculture, mainly focussing on estimation models. The flow of chapters takes the readers in a smooth path, in understanding climate and weather and impact of climate change, and gradually proceeds towards downscaling techniques and then finally towards development of principal component regression models and applying the same for the crop yield estimation.

Statistical science --- Mathematics --- Meteorology. Climatology --- General ecology and biosociology --- Applied physical engineering --- Water supply. Water treatment. Water pollution --- Environmental protection. Environmental technology --- Engineering sciences. Technology --- Agriculture. Animal husbandry. Hunting. Fishery --- PCR (polymerase chain reaction) --- analyse (wiskunde) --- toegepaste wiskunde --- milieukunde --- waterbehandeling --- economie --- landbouw --- statistiek --- waterverontreiniging --- milieubeleid --- wiskunde --- milieutechnologie --- ingenieurswetenschappen --- klimaatverandering --- broeikaseffect --- statistisch onderzoek