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Cytology --- Virology --- Cytologie --- Virologie --- Cytology. --- Virology. --- Cell Biology. --- Celle --- Cellular Biology --- Biologies, Cell --- Biologies, Cellular --- Biology, Cell --- Biology, Cellular --- Cell Biologies --- Cellular Biologies --- Cell biology --- Cellular biology --- Microbiology --- Biology --- Cells --- Cytologists --- Citologia --- Virologia --- Celle.

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Cytology --- Developmental biology --- Cells --- Developmental Biology --- Cytology. --- Developmental biology. --- Cells. --- Developmental Biology. --- Development (Biology) --- Cell biology --- Cellular biology --- Biology, Developmental --- Cell --- Embryonic and Fetal Development --- Growth and Development --- Cell Biology --- Biology --- Growth --- Ontogeny --- Cytologists --- Auxology --- Biologia del desenvolupament --- Citologia --- Cèl·lules

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This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. .

Sistema immunitari --- Sistema immunològic --- Anatomia --- Immunologia --- Cèl·lules immunocompetents --- Medul·la òssia --- Sistema limfàtic --- Immunology. --- Cytology. --- Medicine—Research. --- Biology—Research. --- Cell Biology. --- Biomedical Research. --- Cell biology --- Cellular biology --- Biology --- Cells --- Immunobiology --- Life sciences --- Serology --- Citologia --- Biologia cel·lular --- Biologia --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules --- Cèl·lules animals --- Ultraestructura (Biologia)

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This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.

Microbis --- Hibridació --- Fluorescència --- Citogenètica --- Manuals de laboratori --- Tècniques de laboratori --- Genètica cel·lular --- Citologia --- Genètica --- Citogenètica humana --- Mapatge cromosòmic --- Luminescència --- Radioactivitat --- Immunofluorescència --- Microscòpia de fluorescència --- Efecte Mössbauer --- Enllumenat fluorescent --- Espectroscòpia de fluorescència --- Hibridació genètica --- Hibridització --- Herència (Biologia) --- Millora de les espècies --- Espècies (Biologia) --- Lleis de Mendel --- Microorganismes --- Gèrmens --- Organismes microscòpics --- Microbiologia agrícola --- Acritarcs --- Bacteris --- Microalgues --- Microbiota --- Microorganismes extremòfils --- Microorganismes patògens --- Perífiton --- Probiòtics --- Procariotes --- Protozous --- Virus --- Microbiologia --- Microscòpia --- Hibridisme --- Cytology. --- Microbiology. --- Cell Biology. --- Microbial biology --- Biology --- Microorganisms --- Cell biology --- Cellular biology --- Cells

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This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and characterization of allergen specific T cells, and the role of antigen presenting cells in T cell proliferation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Allergy: Methods and Protocols, Second Edition is a valuable resource for researchers interested in beginning or continuing their research in the study of allergies.

Immunology. --- Allergy. --- Allergology. --- Allergic diseases --- Allergies --- Hypersensitivity --- Hypersensitivity, Immediate --- Immediate allergy --- Immediate hypersensitivity --- Immunologic diseases --- Immunoglobulin E --- Immunobiology --- Life sciences --- Serology --- Al·lèrgia --- Immunologia --- Citologia --- Histologia --- Al·lèrgens --- Transformació genètica --- Manuals de laboratori --- Anatomia microscòpica --- Histologia humana --- Anatomia --- Cèl·lules --- Histologia veterinària --- Histopatologia --- Histoquímica --- Teixits (Histologia) --- Ultraestructura (Biologia) --- Tècniques de laboratori --- Transferència gènica --- Àcids nucleics --- Antígens --- Biologia cel·lular --- Biologia --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules animals --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari --- Al·lèrgies --- Processos al·lèrgics --- Al·lèrgia alimentària --- Al·lèrgia als medicaments --- Al·lèrgia en els infants --- Dermatitis atòpica --- Dermatitis de contacte --- Fotosensibilització (Biologia) --- Urticària --- Histamina