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Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics

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This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.

Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Vectors genètics --- Manuals de laboratori --- Protocols clínics --- Plans d'actuació mèdica --- Protocols en medicina --- Protocols de diagnòstic clínic --- Protocols mèdics --- Protocols terapèutics --- Medicina clínica --- Històries clíniques --- Protocols d'infermeria --- Tècniques de laboratori --- Vectors de clonatge --- Clonatge molecular --- Plasmidis --- Virus --- ADN recombinant --- Expressió gènica

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This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Genetics. --- Genomics. --- Genetics and Genomics. --- Genome research --- Genomes --- Molecular genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Research --- Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics

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Groundbreaking, comprehensive, and developed by a panel of leading international experts in the field, Textbook of Assisted Reproduction provides a multidisciplinary overview of the diagnosis and management of infertility, which affects 15% of all couples around the world. The book aims to cover all aspects of assisted reproduction. Particular attention is given to topics such as the assessment of infertile couples; assisted reproductive techniques (ARTs) including ovulation induction, intra uterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (clinical and laboratory aspects); reproductive genetics; and obstetric and perinatal outcomes.

Gynecology . --- Gynecology. --- Gynaecology --- Medicine --- Generative organs, Female --- Diseases --- Human reproductive technology. --- Assisted conception --- Assisted human reproduction --- Assisted human reproductive technology --- Conception --- Human assisted reproduction --- Human assisted reproductive technology --- Human reproduction --- Medical technology --- Reproductive technology --- Technological innovations --- Reproducció humana assistida --- Reproducció assistida humana --- Tècniques de reproducció assistida --- Tecnologia reproductiva humana --- Reproducció assistida --- Reproducció humana --- Clonatge humà --- Gestació subrogada --- Turisme reproductiu

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This book provides a comprehensive overview of the basic and advanced metabolic engineering technologies used to generate natural metabolites and industrially important biomolecules. Metabolic engineering has the potential to produce large quantities of valuable biomolecules in a renewable and sustainable manner by extending or modifying biosynthetic pathways in a wide range of organisms. It has been successfully used to produce chemicals, drugs, enzymes, amino acids, antibiotics, biofuels, and industrially important pharmaceuticals. The book comprehensively reviews the various metabolites detection, extraction and biosensors and the metabolic engineering of microbial strains for the production of industrially useful enzymes, proteins, organic acids, vitamins and antibiotics, therapeutics, chemicals, and biofuels. It also discusses various genetic engineering and synthetic biology tools for metabolic engineering. In closing, the book discusses ethical, patenting and regulatory issues in the metabolic engineering of microbes. This book is a valuable source not only for beginners in metabolic engineering, but also students, researchers, biotechnology and metabolic engineering based company.

Biomedical engineering. --- Genetic engineering. --- Microbiology. --- Biochemical engineering. --- Bioethics. --- Computer simulation. --- Biomedical Engineering/Biotechnology. --- Genetic Engineering. --- Biochemical Engineering. --- Simulation and Modeling. --- Computer modeling --- Computer models --- Modeling, Computer --- Models, Computer --- Simulation, Computer --- Electromechanical analogies --- Mathematical models --- Simulation methods --- Model-integrated computing --- Biology --- Biomedical ethics --- Life sciences --- Life sciences ethics --- Science --- Bio-process engineering --- Bioprocess engineering --- Biochemistry --- Biotechnology --- Chemical engineering --- Microbial biology --- Microorganisms --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Moral and ethical aspects --- Enginyeria biomèdica --- Enginyeria genètica --- Microbiologia --- Biologia --- Bacteriologia --- Ecologia microbiana --- Genètica microbiana --- Microbiologia agrícola --- Microbiologia aquàtica --- Microbiologia dels sòls --- Microbiologia mèdica --- Microbiologia molecular --- Protozous --- Química microbiològica --- Virologia --- Microorganismes --- Microscòpia --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics --- Enginyeria clínica --- Enginyeria mèdica --- Bioenginyeria --- Biofísica --- Enginyeria --- Medicina --- Electrònica mèdica --- Enginyeria de teixits --- Materials biomèdics --- Aparells i instruments mèdics