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The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (for example, histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein: DNA interactions "in vivo" using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei "in vitro".
Chromosomes. --- Chromosome theory --- Cell nuclei --- Crossing over (Genetics) --- Cytotaxonomy --- Genetics --- Karyokinesis --- Linkage (Genetics) --- Molecular biology --- molecular biology --- gisten --- PCR (polymerase chain reaction) --- genetica --- moleculaire biologie
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DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.
DNA viruses. --- Deoxyribonucleic acid viruses --- Viruses, DNA --- Viruses --- General virology --- PCR (polymerase chain reaction) --- mutageniteit --- virologie --- DNA (deoxyribonucleic acid) --- moleculaire biologie --- spectrometrie --- Dna virus infections --- Dna viruses --- Dna viruses, isolation & purification. --- Virology. --- Isolation & purification. --- Physiology.
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Molecular biology --- Computer. Automation --- Bioinformatics --- Data processing --- bioinformatica --- internet --- genoom --- database --- pcr (polymerase chain reaction) --- dna --- fylogenetica --- Contains audio-visual material --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Computational biology. --- Molecular biology - Data processing
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Tegenwoordig kunnen evolutiebiologen de stamboom van de evolutie vrijwel vanaf de wortels reconstrueren via de analyse van DNA. Lewin laat zien hoe moderne genetica leidt tot nieuwe inzichten in evolutionaire veranderingen.
574 --- DNA --- Evolutieleer --- Genetica --- Moleculaire antropologie --- Moleculaire ecologie --- Moleculen --- Morfologie --- Afstammingsleer - Evolutieleer --- moleculaire biologie --- genetica --- 575.852 --- #WPLT:syst --- evolutieleer --- 57.05 --- 572 --- 575.8 --- DNA deoxyribonucleic acid --- PCR polymerase chain reaction --- erfelijkheid --- eukaryoten --- evolutie --- mutaties --- planten --- prokaryoten --- pseudogenen --- variabiliteit biologie --- verwantschap --- 041206.jpg --- Moleculaire biologie --- Biomacromolecules. Molecular evolution --- afstammingsleer, evolutieleer --- History of medicine --- Paleopathology --- Chemische evolutie --- Evolutie : moleculen --- Anthropology. --- Paleopathology. --- Evolutieleer. --- 20th century. --- Netherlands. --- 575.852 Biomacromolecules. Molecular evolution --- antropologie --- biologie --- #WNAT:didaktiek --- classificatie --- dieren --- ecologie --- Molecular biology. --- Evolution. Phylogeny --- Molecular biology --- 54
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Over the last few years, the subject of food authenticity and food fraud has received increasing attention from consumers and other stakeholders, such as government agencies and policymakers, control labs, producers, industry, and the research community. Among the different approaches aiming to identify, tackle, and/or deter fraudulent practices in the agri-food sector, the development of new, fast, and accurate methodologies to evaluate food authenticity is of major importance. This book, entitled “Target and Non-Target Approaches for Food Authenticity and Traceability”, gathers original research and review papers focusing on the development and application of both targeted and non-targeted methodologies applied to verify food authenticity and traceability. The contributions regard different foods, among which some are frequently considered as the most prone to adulteration, such as olive oil, honey, meat, and fish. This book is intended for readers aiming to enrich their knowledge through reading contemporary and multidisciplinary papers on the topic of food authentication.
Technology: general issues --- COIBar–RFLP (cytochrome oxidase I barcode–restriction fragment length polymorphism) --- seafood --- fraud --- DNA barcoding --- food authenticity --- food adulteration --- food fraud --- donkey --- cytochrome b --- real-time PCR --- meat products --- honey --- regional origin --- chemometric analysis --- mineral content --- Montenegro --- Sepia --- common cuttlefish --- Sepia officinalis --- real-time PCR (Polymerase Chain Reaction) --- species identification --- food authentication --- COI (Cytochrome Oxidase I) --- Olea europaea var Sylvestris --- oleaster --- olive --- olive oil --- adulteration --- SNP --- DNA --- virgin olive oil --- quality --- volatile compounds --- sensory analysis --- chemometrics --- anti food fraud --- Curcuma longa --- DNA markers --- SYBR-GREEN real-time PCR --- Zea mays --- pasta --- Triticum aestivum --- Triticum durum --- genetic traceability --- digital PCR --- semolina --- species --- truffle --- Tuber spp. --- species differentiation --- near-infrared spectroscopy --- red deer --- roe deer --- water deer --- multiplex PCR --- capillary electrophoresis --- perilla --- sesame --- geographic origin --- metabolomics --- multivariate analysis --- metabolite profiling --- quantification --- chicken --- guinea fowl --- pheasant --- quail --- turkey --- authentication --- authenticity --- chemometric --- fish --- origin --- meat --- milk --- spectroscopy --- 1H-NMR --- GC-MS --- HPLC-UV/VIS --- protein hydrolysate --- free amino acid contents --- ProHydrAdd --- monofloral honey --- direct analysis in real time (DART) --- high resolution mass spectrometry (HRMS) --- geographical origin --- Ginkgo biloba --- plant infusions --- real-time polymerase chain reaction --- DNA extraction --- opium poppy --- seed --- pollen grains --- bakery product --- oil --- PCR --- Salmo salar L. --- fatty acids --- mislabeling --- machine learning --- n/a --- COIBar-RFLP (cytochrome oxidase I barcode-restriction fragment length polymorphism)
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Genomics --- Gene amplification --- Polymerase chain reaction --- Gene amplification. --- Polymerase chain reaction. --- Réaction en chaîne de la polymérase --- Redondance génétique --- Amplification génique --- Polymerase Chain Reaction --- Genetic Engineering --- Nucleic Acid Amplification Techniques --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Genetic Counseling --- Genetic Phenomena --- Amplification Technics, Nucleic Acid --- Amplification Techniques, Nucleic Acid --- DNA Amplification Technics --- Nucleic Acid Amplification Technics --- Nucleic Acid Amplification Test --- Nucleic Acid Amplification Tests --- RNA Amplification Technics --- Technics, Nucleic Acid Amplification --- Techniques, Nucleic Acid Amplification --- DNA Amplification Techniques --- RNA Amplification Techniques --- Amplification Technic, DNA --- Amplification Technic, RNA --- Amplification Technics, DNA --- Amplification Technics, RNA --- Amplification Technique, DNA --- Amplification Technique, RNA --- Amplification Techniques, DNA --- Amplification Techniques, RNA --- DNA Amplification Technic --- DNA Amplification Technique --- RNA Amplification Technic --- RNA Amplification Technique --- Technic, DNA Amplification --- Technic, RNA Amplification --- Technics, DNA Amplification --- Technics, RNA Amplification --- Technique, DNA Amplification --- Technique, RNA Amplification --- Techniques, DNA Amplification --- Techniques, RNA Amplification --- Gene Amplification --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Biotechnology --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Anchored Polymerase Chain Reaction --- Inverse Polymerase Chain Reaction --- Nested Polymerase Chain Reaction --- Anchored PCR --- Inverse PCR --- Nested PCR --- PCR --- PCR, Anchored --- PCR, Inverse --- PCR, Nested --- Polymerase Chain Reactions --- Reaction, Polymerase Chain --- Reactions, Polymerase Chain --- Ligase Chain Reaction --- methods --- Nucleic Acid Amplification Technic --- Nucleic Acid Amplification Technique --- GENE AMPLIFICATION --- GENOMICS --- POLYMERASE CHAIN REACTION --- GENOMIC LIBRARY --- PCR (POLYMERASE CHAIN REACTION) --- GENE EXPRESSION --- LABORATORY MANUALS
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