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For a sustainable future, the need to use renewable sources to produce electricity is inevitable. Some of these sources—particularly the widely available solar power—are weather-dependent; therefore, utility-scale energy storage will be more and more important. These solar and wind power fluctuations range from minutes (passing cloud) to whole seasons (winter/summer differences). Short-term storage can be solved (at least theoretically) with batteries; however, seasonal storage—due to the amount of storable energy and the self-discharging of some storage methods—is still a challenge to be solved in the near future. We believe that biological Power-to-Methane technology—especially combined with biogas refinement—will be a significant player in the energy storage market within less than a decade. The technology produces high-purity methane, which can be considered—by using green energy and carbon dioxide of biological origin—as a Renewable Natural Gas, or RNG. The ease of storage and use of methane, as well as the effective carbon-freeness, can make it a competitor for batteries or hydrogen-based storage, especially for storage times exceeding several months.

seasonal energy storage --- power-to-methane --- wastewater treatment plants --- techno-economic assessment --- power-to-gas --- regulation --- energy storage --- biogas --- biomethane --- disruptive technology --- decarbonization --- innovation --- Power-to-Gas --- Power-to-Fuel --- P2M --- P2G --- P2F --- biomethanization --- biomethanation --- competitiveness --- hydrogen utilization --- Hungary --- Power-to-X --- Power-to-Hydrogen --- Power-to-Methane --- hydrogen --- methanation --- sector coupling --- sectoral integration --- energy transition --- eFuels --- electric fuels --- 100% renewable energy scenarios --- thermophilic biogas --- fed-batch reactor --- Methanothermobacter --- metagenome --- starvation --- H2 and CO2 conversion --- methane --- acetate --- n/a

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Process monitoring and control are fundamental to all processes; this holds especially for bioprocesses, due to their complex nature. Usually, bioprocesses deal with living cells, which have their own regulatory systems. It helps to adjust the cell to its environmental condition. This must not be the optimal condition that the cell needs to produce whatever is desired. Therefore, a close monitoring of the cell and its environment is essential to provide optimal conditions for production. Without measurement, no information of the current process state is obtained. In this book, methods and techniques are provided for the monitoring and control of bioprocesses. From new developments for sensors, the application of spectroscopy and modelling approaches, the estimation and observer implementation for ethanol production and the development and scale-up of various bioprocesses and their closed loop control information are presented. The processes discussed here are very diverse. The major applications are cultivation processes, where microorganisms were grown, but also an incubation process of bird’s eggs, as well as an indoor climate control for humans, will be discussed. Altogether, in 12 chapters, nine original research papers and three reviews are presented.

bioenvironmental control --- model-predictive controller --- zonal controlling --- dynamic modelling --- flotation --- bioleaching --- frother --- mixed culture --- machine learning --- Raman spectroscopy --- downstream processing --- chromatography --- flow cell --- extended Kalman filter --- biotechnological processes --- bioreactor control --- specific growth rate control --- batch-to-batch reproducibility --- thermal sensation --- thermal comfort --- machine-learning --- prediction --- adaptive controlling --- thermal growth curve --- temperature modeling --- thermoregulation --- monitoring and control --- bioprocess engineering --- calorimetry --- biocontrol agent --- Bacillus subtilis natto --- isolation --- molecular identification --- medium optimization --- antimicrobial activity --- bactericides --- spectral analyses --- biological selenate reduction --- electron donor competition --- nitrate --- perchlorate --- sequencing batch --- Pseudomonas --- siderophores --- antagonism --- batch fermentation --- exponential fed-batch fermentation --- bio-friendly formulations --- biocontrol --- nonlinear state estimation --- geometric observer --- bioreactor --- continuous system --- model-based sensor --- well-defined macromolecules --- sequence-defined macromolecules --- sequence-defined polymers --- conjugated oligomers --- oligo(arylene ethynylene)s --- biosensors --- sensors --- process monitoring --- bioprocess monitoring and control --- signal noise management --- dielectric spectroscopy --- PAT --- microbial bioprocessing --- n/a

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The use of biocatalysts, including enzymes and metabolically engineered cells, has attracted a great deal of attention in the chemical and bio-industry, because biocatalytic reactions can be conducted under environmentally-benign conditions and in more sustainable ways. The catalytic efficiency and chemo-, regio-, and stereo-selectivity of enzymes can be enhanced and modulated using protein engineering. Metabolic engineering seeks to enhance cellular biosynthetic productivity of target metabolites via controlling and redesigning metabolic pathways using multi-omics analysis, genome-scale modeling, metabolic flux control, and reconstruction of novel pathways. The aim of this book is to cover the recent advances in biocatalysis and metabolic engineering for biomanufacturing of biofuels, chemicals, biomaterials, and pharmaceuticals. Reviews and original research articles on the development of new strategies to improve the catalytic efficiency of enzymes, biosynthetic capability of cell factories, and their applications in production of various bioproducts and chemicals are included.

n/a --- fluorescein diacetate --- Methylosinus sporium strain 5 --- soluble methane monooxygenase --- tunable expression system --- FTIR spectroscopy --- mevalonate kinase 1 --- poly(ethylene glycol) --- tetraethylene glycol --- review --- mevalonate (MVA) --- biofilm --- 5-hydroxymethylfurfural --- polymer functionalization --- microbial production --- microbial cell factory --- bio-hydrogen --- redox enzymes --- specific recognition --- fed-batch fermentation --- monoterpene --- Vitreoscilla --- Pvgb --- bioreactor --- 3-hydroxypropionic acid --- cascade reactions --- synthetic biology --- aerobic methane bioconversion --- starch hydrolysis --- CYP153A --- MEP pathway --- cross-linked enzyme aggregate --- interfacial activation --- expression vectors --- Combi-CLEAs --- polyethyleneimine --- bovine serum albumin --- polyurethane foam --- 12-hydroxydodecanoic acid --- MEV pathway --- amyloglucosidase --- total enzymatic activity --- Nylon 12 --- biocatalytic reaction --- Myceliophthora --- whole-cell biotransformation --- magnetic nanoparticles --- lipase immobilization --- Methanosarcina mazei --- biocatalysis --- acetate --- vgb --- C–H activation --- artificial self-sufficient P450 --- whole cell --- bioplastics --- Corynebacterium glutamicum --- chemicals addition --- enzyme modulation --- Eversa --- enzyme stabilization --- biocatalysts --- prokaryotic microbial factory --- synthetic metabolic pathways --- mannose --- immobilization --- (?)-?-bisabolol --- hydrogenase --- O2 activation --- string film reactor --- fatty acid synthesis --- ?-aminododecanoic acid --- transesterification --- mass transfer performance --- dodecanoic acid --- metabolic engineering --- glyoxal oxidase --- small molecules --- Candida antarctica Lipase B --- C-H activation