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Genetic vectors --- -Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- Handbooks, manuals, etc --- genetic engineering --- genotypes --- Cloning vectors --- Vecteur

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The development of technologies that allow targeting of specific cells has progressed substantially in recent years for several types of vectors, particularly viral vectors, which have been used in 70% of gene therapy clinical trials. Particular viruses have been selected as gene delivery vehicles because of their capacities to carry foreign genes and their ability to efficiently deliver these genes associated with efficient gene expression. This book is designed to present the most recent advances in viral gene therapy

Gene therapy. --- Genetic vectors. --- Viral genetics. --- Virus genetics --- Viruses --- Microbial genetics --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- Therapy, Gene --- Genetic engineering --- Therapeutics --- Genetics --- Medical genetics

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Genetic manipulation of the adult mammalian nervous system is one of the most exciting areas in contemporary neurobiology. The explosive growth of this field has been facilitated by harnessing the power of viruses to transfer genetic material into mammalian cells.Viral Vectors: Gene Therapy and Neuroscience Applications represents the first comprehensive review of viral vector applications to the nervous system by leaders in virology, molecular neurobiology, neuroanatomy, and developmental neurobiology. It serves both as a source of fundamental information for those newly interested

Central nervous system --- Genetic vectors. --- Viruses. --- Genetic vectors --- Microorganisms --- Mobile genetic elements --- Extrachromosomal DNA --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- Nervous system, Central --- Nervous system --- Diseases --- Gene therapy.

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Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results. Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.

Plasmids --- Escherichia coli --- Genetic vectors --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- E. coli (Bacterium) --- Escherichia --- Paragenes --- Cytoplasmic inheritance --- Mobile genetic elements --- Extrachromosomal DNA --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition

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The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.   Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

Medical microbiology, virology, parasitology --- Molecular biology --- Gene therapy --- Genetic vectors --- Transfection --- Viral genetics --- Virus-vector relationships --- Organisms --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Viruses --- Genetic Vectors --- Medicine --- Health & Biological Sciences --- Pathology --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Animal Viruses --- Zoophaginae --- Animal Virus --- Virus --- Virus, Animal --- Viruses, Animal --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Vector-virus relationships --- Virus genetics --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Therapy, Gene --- Genetics --- Human genetics. --- Medical virology. --- Gene therapy. --- Human Genetics. --- Virology. --- Gene Therapy. --- Medical microbiology --- Virology --- Virus diseases --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology